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a647 conjugated hpi2 antibody  (Novus Biologicals)


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    Novus Biologicals a647 conjugated hpi2 antibody
    A647 Conjugated Hpi2 Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/a647+conjugated+hpi2+antibody/HPi2+Antibody+(HIC1-2B4%2E2B)+%5BAlexa+Fluor%C2%AE+647%5D/pm33205073-178-15-18
    Average 91 stars, based on 1 article reviews
    a647 conjugated hpi2 antibody - by Bioz Stars, 2026-09
    91/100 stars

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    Centrifugation:

    Article Title: Human T Cells Expressing a CD19 CAR-T Receptor Provide Insights into Mechanisms of Human CD19-Positive β Cell Destruction
    Article Snippet: .. After centrifugation, cells were resuspended in PBS with 1% BSA, and stained with 1:100 diluted A647-conjugated HPi2 antibody (Novus Biologicals NBP1-18946AF647) and DyLight 488 conjugated HPx1 Antibody (Novus Biologicals NBP1-18951G), for 30 min at 4°C. .. After washing, HPi2 positive and HPx1 negative endocrine cells were sorted with a FACSAria (BD Biosciences) cell sorter, and about 2000 cells were plated in 0.1 mL PIM medium supplemented with 10 μM Rho kinase (ROCK) inhibitor Y-27632 to each well of 96-well round bottom ultra-low attachment plates (Sigma Aldrich, CLS7007) to form aggregated pseudoislets.

    Article Title: Human T Cells Expressing a CD19 CAR-T Receptor Provide Insights into Mechanisms of Human CD19-Positive β Cell Destruction.
    Article Snippet: .. After centrifugation, cells were resuspended in PBS with 1% BSA, and stained with 1:100 diluted A647-conjugated HPi2 antibody (Novus Biologicals NBP118946AF647) and DyLight 488 conjugated HPx1 Antibody (Novus Biologicals NBP1-18951G), for 30 min at 4 C. After washing, HPi2 positive and HPx1 negative endocrine cells were sorted with a FACSAria (BD Biosciences) cell sorter, and about 2000 cells were plated in 0.1 mL PIM medium supplemented with 10 mM Rho kinase (ROCK) inhibitor Y-27632 to each well of 96-well round bottom ultra-low attachment plates (Sigma Aldrich, CLS7007) to form aggregated pseudoislets. .. Cell Reports Medicine 1, 100097, September 22, 2020 e4 Human PBMC isolation, T cell enrichment, and preparation of CAR-T cells PBMCs were isolated from buffy coat samples (Research blood components, item number 002) from healthy volunteers with Ficollpaque plus (GE Healthcare) centrifugation.

    Staining:

    Article Title: Human T Cells Expressing a CD19 CAR-T Receptor Provide Insights into Mechanisms of Human CD19-Positive β Cell Destruction
    Article Snippet: .. After centrifugation, cells were resuspended in PBS with 1% BSA, and stained with 1:100 diluted A647-conjugated HPi2 antibody (Novus Biologicals NBP1-18946AF647) and DyLight 488 conjugated HPx1 Antibody (Novus Biologicals NBP1-18951G), for 30 min at 4°C. .. After washing, HPi2 positive and HPx1 negative endocrine cells were sorted with a FACSAria (BD Biosciences) cell sorter, and about 2000 cells were plated in 0.1 mL PIM medium supplemented with 10 μM Rho kinase (ROCK) inhibitor Y-27632 to each well of 96-well round bottom ultra-low attachment plates (Sigma Aldrich, CLS7007) to form aggregated pseudoislets.

    Article Title: Human T Cells Expressing a CD19 CAR-T Receptor Provide Insights into Mechanisms of Human CD19-Positive β Cell Destruction.
    Article Snippet: .. After centrifugation, cells were resuspended in PBS with 1% BSA, and stained with 1:100 diluted A647-conjugated HPi2 antibody (Novus Biologicals NBP118946AF647) and DyLight 488 conjugated HPx1 Antibody (Novus Biologicals NBP1-18951G), for 30 min at 4 C. After washing, HPi2 positive and HPx1 negative endocrine cells were sorted with a FACSAria (BD Biosciences) cell sorter, and about 2000 cells were plated in 0.1 mL PIM medium supplemented with 10 mM Rho kinase (ROCK) inhibitor Y-27632 to each well of 96-well round bottom ultra-low attachment plates (Sigma Aldrich, CLS7007) to form aggregated pseudoislets. .. Cell Reports Medicine 1, 100097, September 22, 2020 e4 Human PBMC isolation, T cell enrichment, and preparation of CAR-T cells PBMCs were isolated from buffy coat samples (Research blood components, item number 002) from healthy volunteers with Ficollpaque plus (GE Healthcare) centrifugation.



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